f nucleatum subsp polymorphum  (ATCC)

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Proteomic phylogeny of the F. nucleatum bacteriophages. The monophyletic cluster of the Latrobeviruses is highlighted in the dotted green rectangle while the FNU4 bacteriophage is shown in the dotted purple rectangle.

Article Snippet: We isolated and characterized three bacteriophages targeting F. nucleatum subsp. polymorphum ( Fnp ) ATCC 10953 and clinical strains of F. nucleatum (named 0548, 4578: both Fnp ; and 2256-unclassified subspecies).

Techniques:

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Bacteriophage FNU4 alignment with the contig of the host subspecies unclassified F. nucleatum strain 2256 draft genome.

Article Snippet: We isolated and characterized three bacteriophages targeting F. nucleatum subsp. polymorphum ( Fnp ) ATCC 10953 and clinical strains of F. nucleatum (named 0548, 4578: both Fnp ; and 2256-unclassified subspecies).

Techniques:

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Location of protospacers targeted by F. nucleatum strain 2256 CRISPR system, that are found in the bacteriophage FNU4. The putative genes are represented by packaging (black), hypothetical (grey), head and capsid (green), lysis (red), tail (blue), transport and metabolism (yellow), and DNA manipulation (pink) (A). Genome maps of draft genomes of two of the F. nucleatum hosts for bacteriophages. In both F. nucleatum strain 2256 (B) and Fnp ATCC 10953 (C), the genome maps consist of tracks that are labeled from outside to center as track 1: prophage in dark green; tracks 2 & 3 mobile genetic elements including sites for replication (red), integration or excision (light green), defence-related genes (purple) and other transfer genes (olive green). Track 4 indicates the GC content graph, while the size of the genome is indicated by the labels on the innermost track. The bacteriophage FNU4 has homology to the region from 0.4 Mbp to 0.8 Mbp in the F. nucleatum strain 2256 map. Kernel density estimation (KDE) plots showing the uneven clustering of defence system loci across the genomes of F. nucleatum strains 2256 (D) and Fnp ATCC 10953 (E).

Article Snippet: We isolated and characterized three bacteriophages targeting F. nucleatum subsp. polymorphum ( Fnp ) ATCC 10953 and clinical strains of F. nucleatum (named 0548, 4578: both Fnp ; and 2256-unclassified subspecies).

Techniques: CRISPR, Lysis, Labeling

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Mono- and dual-species biofilms imaged via confocal microscopy. F. nucleatum ( Fnp) (green) and P. gingivalis ATCC 33277 ( Pg ) (red) prestained and imaged before biofilm formation (top panel); following biofilm formation (middle panel); biofilms treated with Fnp -specific bacteriophage FNU2 (bottom panel). The biofilms were cultured for up to 48 h (A). 3-D confocal image stack showing (i) attachment of Fnp (green) to cover the slip side and Pg (red) overlaying the Fnp to form a dual-species biofilm. Treatment with (ii) the lytic FNU2 bacteriophage and (iii) the lytic FNU3 bacteriophage disrupted approximately 75% of the biofilm mass. The biofilms were cultured for up to 4 days (B). Quantification of the biofilm-forming capacity of Fnp and P. gingivalis , and their disruption by the Fnp bacteriophages FNU2 and FNU3. The three panels represent Fnp ATCC 10953 mono-species biofilms (C), dual-species biofilms of Fnp ATCC 10953 and P . gingivalis ATCC 33277 (D) and P. gingivalis ATCC 33277 mono-species biofilm (E). Heat-killed F. nucleatum was boiled at 100 ° C for 1 h before being added to P . gingivalis ATCC 33277 biofilm.

Article Snippet: We isolated and characterized three bacteriophages targeting F. nucleatum subsp. polymorphum ( Fnp ) ATCC 10953 and clinical strains of F. nucleatum (named 0548, 4578: both Fnp ; and 2256-unclassified subspecies).

Techniques: Confocal Microscopy, Cell Culture, Disruption

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Proteomic phylogeny of the F. nucleatum bacteriophages. The monophyletic cluster of the Latrobeviruses is highlighted in the dotted green rectangle while the FNU4 bacteriophage is shown in the dotted purple rectangle.

Article Snippet: F. nucleatum (ATCC 10953) was cultured for 48 h, and 100 μL of approximately 1 × 10 8 CFU mL −1 F. nucleatum in the exponential growth phase was added to each well.

Techniques:

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Bacteriophage FNU4 alignment with the contig of the host subspecies unclassified F. nucleatum strain 2256 draft genome.

Article Snippet: F. nucleatum (ATCC 10953) was cultured for 48 h, and 100 μL of approximately 1 × 10 8 CFU mL −1 F. nucleatum in the exponential growth phase was added to each well.

Techniques:

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Location of protospacers targeted by F. nucleatum strain 2256 CRISPR system, that are found in the bacteriophage FNU4. The putative genes are represented by packaging (black), hypothetical (grey), head and capsid (green), lysis (red), tail (blue), transport and metabolism (yellow), and DNA manipulation (pink) (A). Genome maps of draft genomes of two of the F. nucleatum hosts for bacteriophages. In both F. nucleatum strain 2256 (B) and Fnp ATCC 10953 (C), the genome maps consist of tracks that are labeled from outside to center as track 1: prophage in dark green; tracks 2 & 3 mobile genetic elements including sites for replication (red), integration or excision (light green), defence-related genes (purple) and other transfer genes (olive green). Track 4 indicates the GC content graph, while the size of the genome is indicated by the labels on the innermost track. The bacteriophage FNU4 has homology to the region from 0.4 Mbp to 0.8 Mbp in the F. nucleatum strain 2256 map. Kernel density estimation (KDE) plots showing the uneven clustering of defence system loci across the genomes of F. nucleatum strains 2256 (D) and Fnp ATCC 10953 (E).

Article Snippet: F. nucleatum (ATCC 10953) was cultured for 48 h, and 100 μL of approximately 1 × 10 8 CFU mL −1 F. nucleatum in the exponential growth phase was added to each well.

Techniques: CRISPR, Lysis, Labeling

Journal: Journal of Oral Microbiology

Article Title: Characterisation of novel Fusobacterium nucleatum bacteriophages and their efficacy in disrupting pathogenic dual-species biofilms

doi: 10.1080/20002297.2025.2584952

Figure Lengend Snippet: Mono- and dual-species biofilms imaged via confocal microscopy. F. nucleatum ( Fnp) (green) and P. gingivalis ATCC 33277 ( Pg ) (red) prestained and imaged before biofilm formation (top panel); following biofilm formation (middle panel); biofilms treated with Fnp -specific bacteriophage FNU2 (bottom panel). The biofilms were cultured for up to 48 h (A). 3-D confocal image stack showing (i) attachment of Fnp (green) to cover the slip side and Pg (red) overlaying the Fnp to form a dual-species biofilm. Treatment with (ii) the lytic FNU2 bacteriophage and (iii) the lytic FNU3 bacteriophage disrupted approximately 75% of the biofilm mass. The biofilms were cultured for up to 4 days (B). Quantification of the biofilm-forming capacity of Fnp and P. gingivalis , and their disruption by the Fnp bacteriophages FNU2 and FNU3. The three panels represent Fnp ATCC 10953 mono-species biofilms (C), dual-species biofilms of Fnp ATCC 10953 and P . gingivalis ATCC 33277 (D) and P. gingivalis ATCC 33277 mono-species biofilm (E). Heat-killed F. nucleatum was boiled at 100 ° C for 1 h before being added to P . gingivalis ATCC 33277 biofilm.

Article Snippet: F. nucleatum (ATCC 10953) was cultured for 48 h, and 100 μL of approximately 1 × 10 8 CFU mL −1 F. nucleatum in the exponential growth phase was added to each well.

Techniques: Confocal Microscopy, Cell Culture, Disruption